Journal: bioRxiv

Article Title: EZ Clear for simple, rapid, and robust mouse whole organ clearing

doi: 10.1101/2022.01.12.476113

Figure Lengend Snippet: (A) Procedure for EZ Clear tissue processing. (B) Comparison of volume changes of adult mouse brains (9 weeks old) and bright field representative images of fixed in 4% PFA and not cleared, or fixed and cleared using EZ Clear, Fast 3D, 3DISCO, and X-CLARITY cleared brains. (C) Adult mouse organs (eye, heart, lung, liver, pancreas, kidney, and testis) cleared by EZ Clear protocol. (D-F) Volume rendering of wholemount, 4-month-old Thy1-EGFP-M mouse brain cleared with EZ Clear and imaged by LSFM across the total imaging depth of 5 mm, dorsal to ventral at (D) dorsal view and (E and F) sectioned digitally at the transverse (coronal) axis. (G – L) Lightsheet imaging of the right or left hemisphere of a mouse brain perfused with Evans blue dye and then cleared by EZ Clear (right hemisphere, G-I) and Fast 3D (left hemisphere, J-L) shows that EZ Cleared tissue has a greater imaging depth with cleaner signal and less light scattering.

Article Snippet: The refractive index of the Fast 3D clear solution was measured on refractometer (Atago, PAL-RI 3850) and the RI was between 1.511 to 1.513.

Techniques: Imaging

Journal: bioRxiv

Article Title: EZ Clear for simple, rapid, and robust mouse whole organ clearing

doi: 10.1101/2022.01.12.476113

Figure Lengend Snippet: (A) Schematic of required steps and time for tissue clearing with X-CLARITY, Fast 3D, 3DISCO, and EZ Clear. (B) Representative images of brains cleared by X-CLARITY, Fast 3D, 3DISCO, and EZ Clear after 4% PFA fixation, delipidation, and RI matching with EZ View (EZ Clear), Fast 3D imaging solution (Fast 3D), DEB (3DISCO), and sRIMS (X-CLARITY). The outlined boundary of the brain from each of the captured brightfield images was used to quantify the total pixel number and thus brain size at each step (n= 4 brains measured for each solution).

Article Snippet: The refractive index of the Fast 3D clear solution was measured on refractometer (Atago, PAL-RI 3850) and the RI was between 1.511 to 1.513.

Techniques: Imaging

Journal: Brachytherapy

Article Title: Quality Comparison between 3-dimensional T2-weighted SPACE and 2-dimensional T2-weighted turbo spin echo magnetic resonance images for the brachytherapy planning evaluation of prostate and periprostatic anatomy

doi: 10.1016/j.brachy.2020.04.001

Figure Lengend Snippet: Scan parameters for 2D T2W TSE and 3D T2W SPACE sequences.

Article Snippet: [ 5 ] However, development of the 3D fast spin echo sequence (such as SPACE, for sampling perfection with application-optimized contrasts by using flip angle evolution, on the Siemens platform) allows pelvic and prostate T2W imaging at sub-millimeter resolution.

Techniques:

Journal: Brachytherapy

Article Title: Quality Comparison between 3-dimensional T2-weighted SPACE and 2-dimensional T2-weighted turbo spin echo magnetic resonance images for the brachytherapy planning evaluation of prostate and periprostatic anatomy

doi: 10.1016/j.brachy.2020.04.001

Figure Lengend Snippet: Mean scores for delineation of prostate and periprostatic anatomical structures and image quality obtained with 2D T2W TSE and 3D T2W SPACE sequences.

Article Snippet: [ 5 ] However, development of the 3D fast spin echo sequence (such as SPACE, for sampling perfection with application-optimized contrasts by using flip angle evolution, on the Siemens platform) allows pelvic and prostate T2W imaging at sub-millimeter resolution.

Techniques: Sequencing

Journal: Brachytherapy

Article Title: Quality Comparison between 3-dimensional T2-weighted SPACE and 2-dimensional T2-weighted turbo spin echo magnetic resonance images for the brachytherapy planning evaluation of prostate and periprostatic anatomy

doi: 10.1016/j.brachy.2020.04.001

Figure Lengend Snippet: Interobserver agreement in ratings of visibility and delineation of the prostate and periprostatic anatomical structures.

Article Snippet: [ 5 ] However, development of the 3D fast spin echo sequence (such as SPACE, for sampling perfection with application-optimized contrasts by using flip angle evolution, on the Siemens platform) allows pelvic and prostate T2W imaging at sub-millimeter resolution.

Techniques: Sequencing

Journal: Brachytherapy

Article Title: Quality Comparison between 3-dimensional T2-weighted SPACE and 2-dimensional T2-weighted turbo spin echo magnetic resonance images for the brachytherapy planning evaluation of prostate and periprostatic anatomy

doi: 10.1016/j.brachy.2020.04.001

Figure Lengend Snippet: Comparison of 2D T2W TSE and 3D T2W SPACE images for signal – to- noise ration of normal tissues.

Article Snippet: [ 5 ] However, development of the 3D fast spin echo sequence (such as SPACE, for sampling perfection with application-optimized contrasts by using flip angle evolution, on the Siemens platform) allows pelvic and prostate T2W imaging at sub-millimeter resolution.

Techniques: Comparison, Sequencing

Journal: Brachytherapy

Article Title: Quality Comparison between 3-dimensional T2-weighted SPACE and 2-dimensional T2-weighted turbo spin echo magnetic resonance images for the brachytherapy planning evaluation of prostate and periprostatic anatomy

doi: 10.1016/j.brachy.2020.04.001

Figure Lengend Snippet: Relative contrast ratios between tissue types.

Article Snippet: [ 5 ] However, development of the 3D fast spin echo sequence (such as SPACE, for sampling perfection with application-optimized contrasts by using flip angle evolution, on the Siemens platform) allows pelvic and prostate T2W imaging at sub-millimeter resolution.

Techniques: Sequencing

Journal: Diagnostic and Interventional Radiology

Article Title: Denoising using deep-learning-based reconstruction for whole-heart coronary MRA with sub-millimeter isotropic resolution at 3 T: a volunteer study

doi: 10.5152/dir.2022.21291

Figure Lengend Snippet: Acquisition and reconstruction parameters

Article Snippet: Only for Sub-mm, we employed a novel k-space filling technique for fast image acquisition, a “wheel” technique (k-sampling ratio: 80%) in Fast 3D mode (Canon) for acquiring signals at the center of the k-space in a deformed wheel pattern in the phase encode (y-axis)-slice encode (z-axis) plane.

Techniques: